Search results for "Microsatellite Loci"

showing 4 items of 4 documents

Variation in gene expression within clones of the earthworm Dendrobaena octaedra

2017

Gene expression is highly plastic, which can help organisms to both acclimate and adapt to changing environments. Possible variation in gene expression among individuals with the same genotype (among clones) is not widely considered, even though it could impact the results of studies that focus on gene expression phenotypes, for example studies using clonal lines. We examined the extent of within and between clone variation in gene expression in the earthworm Dendrobaena octaedra, which reproduces through apomictic parthenogenesis. Five microsatellite markers were developed and used to confirm that offspring are genetic clones of their parent. After that, expression of 12 genes was measured…

0106 biological sciences0301 basic medicinegenotypeClone (cell biology)lcsh:Medicineearthworms01 natural sciencesMicrosatellite LociDendrobaena octaedraGenotypeGene expressiongeeniekspressioAnnelidslcsh:ScienceGeneticsMultidisciplinarykloonitPhenotypePhenotypesMicrosatelliteGene CloningResearch ArticleGenotypingBiologyResearch and Analysis Methods010603 evolutionary biologygenotyyppi03 medical and health sciencesExtraction techniquesclonesGene TypesApomixisGeneticsAnimalsOligochaetaparthenogenesisMolecular Biology TechniquesMolecular BiologyGenepartenogeneesita1184lcsh:ROrganismsBiology and Life SciencesParthenogenesisInvertebratesRNA extraction030104 developmental biologygene expressionta1181lcsh:QMicrosatellite RepeatsCloningPLoS ONE
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Effect of number of seed parents on the genetic diversity of artificially regenerated stands of holm oak

2007

Artificial populations of holm oak (Quercus ilex L. s.l.) and their natural seed origin populations selected from Andalusia (Southern Spain) and Sicily (Southern Italy) were analysed with six nuclear microsatellite loci to explore the effect of seed collection methods. Low effective allele numbers (3.07-6.67) and extremely low effective populations sizes (35.4-102.9) were detected in both reforestation stands, probably due to an inappropriate forester seed collection strategy limited to a few mother trees. Analysis of simulated progenies showed that optimal sampling size to obtain seed collections genetically similar to the origin populations should range from 20 to 50 individuals, but coul…

Andalusia microsatellite loci population reforestation seed origin SicilySettore BIO/02 - Botanica Sistematica
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Conservation of genetic diversity in artificially regenerated holm oak (Quercus ilex L.) populations

2004

Reforestation with autochtonous species should take into account the preservation of the variability and the geographical structure of genetic diversity in forest species. In order to provide empirical data, genetic composition of an artificial population of holm oak (Quercus ilex L.) and its natural seed-origin population in Andalusia were analysed with six microsatellite loci. The genetic diversity indices applied show a reduction in the artificial stand if compared with the natural one, probably due to an inappropriate forester seed collection strategy limited to a few trees.

Autochthonous species microsatellite loci Quercus ilex reforestation Sierra de Gador
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Absence of spatial genetic structure in common dentex (Dentex dentex Linnaeus, 1758) in the Mediterranean Sea as evidenced by nuclear and mitochondri…

2018

International audience; The common dentex, Dentex dentex, is a fish species which inhabits marine environments in the Mediterranean and Northeast Atlantic regions. This is an important species from an ecological, economic and conservation perspective, however critical information on its population genetic structure is lacking. Most samples were obtained from the Mediterranean Sea (17 sites) with an emphasis around Corsica (5 sites), plus one Atlantic Ocean site. This provided an opportunity to examine genetic structuring at local and broader scales to provide science based data for the management of fishing stocks in the region. Two mitochondrial regions were examined (D-loop and COI) along…

Gene Flow0106 biological sciences0301 basic medicinePopulation geneticsPopulationZoologyPopulation geneticslcsh:MedicineDNA Mitochondrial010603 evolutionary biology01 natural sciencesMarine fish03 medical and health sciencesMediterranean seaGene FrequencyMediterranean SeaAnimals14. Life underwatereducationlcsh:ScienceAtlantic OceanAllelesPhylogenyIsolation by distanceCell NucleusPanmixiaeducation.field_of_studyMultidisciplinary[SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE]biologylcsh:RGenetic VariationBayes TheoremDentex dentexbiology.organism_classificationMitochondrial DNAMitochondriaPerciformesDentex dentex D-loop COI microsatellite loci Mediterranean Sea Atlantic Ocean.Polymerase chain reactionPhylogeographyPhylogeography[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal geneticsGenetics Population030104 developmental biologyHaplotypesGenetic structurelcsh:QMicrosatellite RepeatsPLoS ONE
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